Evaluation of the efficacy of Gegen Qinlian Decoction and Gegen Qinlian Pills in enhancing the anti-colorectal cancer activity of irinotecan
HU Dingxin
ZHANG Ying
ZHANG Zunjian
XU Fengguo
ZHANG Pei
Abstract：Objective This study aimed to compare the efficacy of Gegen Qinlian Pill(GQP)and Gegen Qinlian De-coction(GQD)in enhancing the activity of Irinotecan(CPT-11)against colorectal cancer(CRC).Methods The in vitro ef-ficacy was first assessed using the human CRC cell line HCT-116.Next,a xenograft mouse model was constructed to evalu-ate the in vivo anti-CRC efficacy.Mice were randomized into six groups(n=8):Model,CPT-11,GQD,GQP,GQD+CPT-11,and GQP+CPT-11.Results The cell experiments demonstrated that GQD synergistically enhanced the inhibitory effect of CPT-11,while the combination of GQP and CPT-11 showed an additive effect.In vivo,both GQD and GQP significantly enhanced the anti-tumor activity of CPT-11,with GQP exhibiting a synergistic effect,as indicated by a combination index(Q value)of 1.18.To further investigate the underlying mechanisms,we used liquid chromatography-mass spectrometry to compare the chemical component differences between GQD and GQP.The results revealed that GQP was less abundant in glycosides but more abundant in aglycones compared to GQD,which may contribute to the distinct efficacies observed be-tween GQD and GQP in vivo.Conclusion This study confirms that both GQD and GQP can enhance the anti-CRC activity of CPT-11,with GQP demonstrating a synergistic effect in vivo.These results suggest a potential new strategy for combining traditional Chinese medicines and western medicines in CRC treatment.
Keywords：Gegen Qinlian DecoctionGegen Qinlian PillIrinotecanColorectal cancerDrug combination
Publication Date：2025-01-27
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 1-7,41 )