Study on"quality-quantity"double standards quality analysis method for traditional chinese medicine Citri Reticulatae Pericarpium based on dual wavelength differential fusion spectrum

MENG Ying; ZHANG Qiang; BAO Yongrui; WANG Shuai; LI Tianjiao; MENG Xiansheng

doi:10.13506/j.cnki.jpr.2024.11.009

Abstract：Objective To establish a qualitative and quantitative double standards control method for Citri Reticulatae Pericarpium,which takes the reference traditional Chinese medicinal materials as control substance of plant drug but does not rely on the quantification of multiple reference materials.Methods High performance liquid chromatography(HPLC)was used to establish a characteristic map of Citri Reticulatae Pericarpium based on the reference medicinal materials.Com-bined with dual wavelength differential fusion technology,the common characteristic peaks of 15 batches of Citri Reticulatae Pericarpium medicinal materials were determined.Based on the similarity between the characteristic peaks of the reference medicinal materials and the tested medicinal materials,the authenticity of the medicinal materials,in other words."quality"was determined.Using mass spectrometry technology to identify the chemical composition of characteristic peaks,selecting"hesperidin"as an internal standard component for accurate quantitative analysis,and using reference substances to rela-tively quantify the chemical composition of other characteristic peaks,distinguishing the quality of Citri Reticulatae Peri-carpium medicinal materials,that is"quantity".ResuIts 9 common characteristic peaks were identified,and 4 chemical components were identified,namely naringin,hesperidin,naringin,and tangerine peel extract.The similarity between 10 bat-ches of Citri Reticulatae Pericarpium and the control medicinal herbs is 0.487～0.556,and the similarity between 5 batches of Citri Reticulatae'Chachi'medicinal herbs is 0.992～0.996.The lower limit of the relative content of characteristic peaks of Citri Reticulatae Pericarpium and Citri Reticulatae'Chachi'medicinal herbs is specified.ConcIusion This method does not rely on multiple reference standards and can accurately and efficiently determine the authenticity and quality of medici-nal materials,providing technical support for the quality control of traditional Chinese medicine.