Study on quality analysis method of Euodiae Fructus based on"quality-quantity"double standard
MIAO Menglan
BAO Yongrui
WANG Shuai
LI Tianjiao
GUAN Xin
MENG Xiansheng
Abstract：Objective To establish a"quality-quantity"double standard control method for Evodia officinalis,which is based on the quality of the control material and does not depend on the quantity of the control material.Methods The characteristic spectrum of Evodia officinalis was established by high performance liquid chromatography(HPLC),and the common peak chemical components were identified by mass spectrometry.The authenticity of Evodia officinalis was deter-mined by evaluating the similarity of the characteristic spectrum of Evodia officinalis and the tested medicinal materials.The relative content of other characteristic peaks was calculated with evodiamine as the reference material,and the lower limit of relative content of characteristic peaks was used to judge the quality of evodiamine,that is,the quantity.ResuIts The char-acteristic map of Euodiae Fructus and the determination method of Euodiae Fructus content were in line with the require-ments of methodology investigation.Three chromatographic peaks were identified,namely limonin,evodiamine and rutae-carpine.The similarity between the tested herbs from different origin and the control herbs of Euodiae Fructus was greater than 0.970.The lower limit of relative content of chemical components in characteristic peak of Euodiae Fructus was defined.ConcIusion This method can distinguish the authenticity of medicinal materials quickly and easily without relying on many reference materials,and achieve the purpose of controlling the quality of medicinal materials of Euodiae Fructus.
Keywords：Euodiae Fructus"Quality-quantity"double standard technologyCharacteristic spectrumEvodiamineQualitative research
Publication Date：2024-11-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1078-1083 )