Study on the mechanism of paeonol delaying airway remodeling in asthma based on TLR4/NF-κB pathway
ZHAO Hui
ZHAO Wei
LI Suxia
Abstract：Objective To investigate the mechanism of paeonol delaying airway remodeling in asthma based on TLR4/NF-κB signaling pathway,to explore the possible mechanism of paeonol in treating asthma.Methods Sprague-dawley rats were randomly divided into normal control group,asthma model group,dexamethasone group,low-dose paeonol group(75 mg·kg-1),middle-dosepaeonol group(150 mg·kg-1)and high-dose paeonol group(300 mg·kg-1).The asthma rat model was established by ovalbumin(OVA)sensitization.The treatment group was treated by aerosol inhalation.The pathological changes of lung tissue were detected by HE staining;The thickness of airway wall was analyzed by computer image analysis;The expression of NF-κB protein was detected by immunohistochemistry,the expression of TLR4 mRNA was detected by RT-PCR.ResuIts Compared with the normal control group,the asthmatic model group showed severe pathological injury and airway remodeling,WAi/Pbm,WAt/Pbm,WAm/Pbm and the expression of TLR4 and NF-κB in lung tissue were increased(P<0.05).After treated with artesunate and paeonol,WAi/Pbm,WAt/Pbm and WAm/Pbm,the expression of TLR4 and NF-κB in lung tissue were decreased(P<0.05).ConcIusion Paeonol can effectively improve airway remodeling in asth-matic rats,and the mechanism may be related to the inhibition of TLR4/NF-κB signaling pathway in the lung tissue of asth-matic rats.
Keywords：PaeonolAirway remodelingToll-like receptor 4Nuclear factor-κB
Publication Date：2024-11-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1067-1071 )