Establishment of HPLC fingerprint and study on the content five constituents for Xiao'er Huatan Zhike Granules
LI Jie
YIN Lihua
CHI Xinru
WU Aiying
YIN Shining
LIU Hongbing
LU Jingguang
Abstract：Objective To establish the fingerprint of Xiao'er Huatan Zhike Granules,and to determine the contents of ephedrine hydrochloride,mulberroside A,cephaeline dihydrochloride,emetine hydrochloride and ipecacuanhin,the quality difference of samples from multiple enterprises was analyzed by chemometrics.Methods High performance liquid chroma-tography(HPLC)and multi-wavelength switching detection were used.The mobile phase is acetonitrile-0.4％phosphoric acid solution and flow rate is 1.0 mL·min-1.Column oven temperature 35 ℃;Similarity,cluster analysis and principal com-ponent analysis were used for evaluation.Results The similarity of twenty-one batches of Xiao'er Huatan Zhike Granules was greater than 0.897,there were eleven common peaks,and the five indexes showed a good linear relationship(r ≥0.999 0).The recoveries were 96.68％～101.90％,and the RSD was less than 2％.Conclusion The established infantile phlegm cough particle fingerprint and 5 kinds of index component content determination method can be used for the quality control and quality evaluation.
Keywords：Xiao'er Huatan Zhike GranulesFingerprintsMulticomponent content determinationChemometrics
Publication Date：2024-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 952-956,1014 )