The effect of harmine on inflammatory injury of lung tissue in MODS rats
YU Wenlong
CAI Yujia
PANG Yuyang
YU Mingyun
ZHANG Na
YANG Xiuhong
LIU Yan
Abstract：Objective To observe the protective effect of harmine(HAM)on lung tissue inflammation injury in rats with multiple organ dysfunction syndrome(MODS).Methods MODS animal model was established and rats were randomly divided into control group,MODS group and HAM group.The lung wet-dry weight ratio and lung coefficient were calculated respectively.Lung tissue morphology was observed by HE staining.The levels of IL-6,IL-10 and TNF-α in serum were detected by ELISA kit.The localized expression of S100 calcium-binding protein A8/A9(S100 A8/A9)was detected by immunohistochemical staining.Western blotting was used to detect the expression of S100 A8/A9,M1 macro-phage marker inducible equivalent of nitric oxide synthase(iNOS),and M2 macrophage marker arginase-1(Arg-1)in lung tissue.Results Compared with MODS group,the ratio of lung wet and dry weight and lung coefficient in HAM group were decreased,inflammatory exudation of alveolar cavity was reduced,pulmonary interstitial hyperemia and edema were al-leviated,serum IL-6 and TNF-α levels were decreased,IL-10 levels were increased,S100 A8/A9 and iNOS expression in lung tissue were decreased,and Arg-1 expression was increased.Conclusion HAM can improve lung tissue injury in MODS rats by inhibiting inflammatory response.
Keywords：Multiple organ dysfunction syndromeHarmineMacrophageInflammationS100 A8/A9
Publication Date：2024-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 948-951,967 )