Metformin reversed acquired resistance to tamoxifen in breast cancer by inhibiting NF-κB signaling pathway
LI Ling
HUANG Xin
LI Yan
HU Mengdi
XU Lu
Abstract：Objective To explore the function and mechanism of metformin in overcoming acquired resistance to tamoxifen in breast cancer.Methods CCK-8 and colony formation assays were conducted to detect cell proliferation.Flow cytometry was employed to detect cell apoptosis.Western blotting was applied to detect the protein expression of cleaved-PARP and NF-κB p65.Immunofluorescence analysis was used to detected the NF-κB p65 nuclear translocation.Results We found that metformin inhibited proliferation and promoted apoptosis of breast cancer cells and acquired resistant cells.Metformin significantly delayed the emergence of acquired resistance to tamoxifen.In addition,metformin could inhibit NF-κB signaling pathway.Conclusion Metformin reversed acquired resistance to tamoxifen in breast cancer via NF-κB signaling pathway.Our study repurposes metformin as a potential candidate for overcoming acquired resistance to tamoxifen.
Keywords：MetforminBreast cancerAcquired resistanceTamoxifenNF-κB
Publication Date：2024-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 943-947 )