Study on the mechanism of Shexiang Xintongning against neointimal hyperplasia after vascular injury in rats based on Rho/Rock pathway
YU Lingqun
GAO Weiping
SHI Mingqian
XIN Xin
WANG Mengyuan
CAO Guiyun
YANG Ying
Abstract：Objective To observe the effect of Shexiang Xintongning(SXN)on intimal hyperplasia after vascular injury in rats and explore possible mechanisms of action.Methods 30 Sprague-Dawley rats were randomly divided into control,model,and SXN groups.The rat model of abdominal aortic vascular injury was prepared by balloon injury method.Af-ter the model was constructed,SXN(200 mg·kg-1)was given by intragastric administration.After administrated with drugs for 14 days,and the serum and abdominal aortic vascular tissues were collected,and the aortic vessels were stained with HE,the contents of SOD,MDA,MCP-1 and GSH-Px in serum were detected,the contents of NO,ET-1,VEGF and IL-1 β in rat vascular tissues were detected by ELISA,the mRNA and protein expressions of Rho,Rock1 and Rock2 were detected by real-time qPCR and Western blotting.Results Compared with control group,the intima hyperplasia of abdominal aorta in model group was increased,the elasticity of some parts of abdominal aorta was weakened and the tube wall was thickened.The injury degree of vascular intima was reduced after SXN treatment.Compared with model group,the activities of SOD and GSH-Px in serum of SXN group were increased,while the activities of MCP-1 and MDA were decreased(P＜0.05).The contents of NO in vascular tissue were increased,and the contents of ET-1,VEGF and IL-1β were decreased(P＜0.05).The expressions of Rho/Rock pathway-related factors Rock1,Rock2 and Rho were decreased(P＜0.05).Conclusion SXN reduces intimal injury in rats with vascular injury,which may be related to its inhibition of Rho/Rock signaling pathway.
Keywords：Rho/Rock pathwayShexiang XintongningIntimal hyperplasiaArterial balloon injury
Publication Date：2024-03-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 232-235 )