Study of the protective effect of Lactic Acid Bacteria Complex Enteric Capsules strengthen Shenshuaining Capsules on renal fibrosis

YANG Hao; SONG Xianrui; ZHANG Lei

doi:10.13506/j.cnki.jpr.2024.02.004

Abstract：Objective To investigate the protective effect of Lactic Acid Bacteria Complex Enteric Capsules(LCC)combined with Shenshuaining Capsules(SSN)on renal fibrosis in the ureteral obstruction(UUO)surgical rat models.Meth-ods LCC were tested.The UUO surgery was performed to establish renal fibrosis model in rat models.After drug combina-tion of LCC and SSN for 21 days,terminal body weight was recorded and the renal coefficient was calculated;BUN,Scr and MAU were detected by biochemical instrument;Kidney tissue morphology and the degree of fibrosis were observed and eval-uated by HE and Masson staining;The expression of α-SMA and Col1 A in kidney tissues was determined by immunohisto-chemistry;The content of IL-6,TNF-α,CRP and TGF-β in renal tissue and the content of D-lactate,endotoxin and diam-ine oxidase(DAO)in serum were detected by ELISA;The mRNA expression of ZO-1,Occludin,Claudin-1,and Claudin-2 in the ileal tissue was determined by Q-PCR.Results Compared to SSN group,the rat models with drug combination of LCC combined with SSN treatment significantly reduced renal coefficient,BUN,Scr and MAU;Increased the body weight;Significantly improved renal tissue morphology and collagen accumulation;Decreased the expression of α-SMA and Col 1 A protein,IL-6,TNF-α,CRP,TGF-p,D-lactate and diamine oxidase(DAO);Increased mRNA expression of ZO-1,Occlu-din,Claudin-1 and decreased mRNA expression of Claudin-2,and ultimately improved renal fibrosis.Conclusion LCC can enhance the improvement of SSN on renal fibrosis,and its effect is mainly related to anti-inflammation,anti-fibrosis,regulation of microflora balance and improvement of intestinal mucosal barrier.Based on the renal-intestinal axis theory,it provides a basis and a new treatment method for the treatment of renal fibrosis.