Chemical composition analysis of the root of Murraya exotica based on UPLC-Q-Orbitrap-MS
SUN Lu
DENG Shiren
XIA Linbo
BIAN Xing
YANG Hao
Abstract：Objective To establish a method for the analysis of the main chemical components in the root of Murraya exotica using UPLC-Q-Orbitrap MS.Methods The analysis was performed on Waters ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.8 μm)chromatographic column,with 0.1%formic acid water(A)-0.1%formic acid acetonitrile(B)as the mobile phase for gradient elution.MS1 and MS2 data of samples were collected in positive and negative ion mode,respective-ly.Results By deducing the fragmentation rule of mass spectrometry,comparing with the reference substance,literature data and mass spectrometry database,63 components were preliminarily identified from the root of M.exotica,mainly inclu-ding coumarins,flavonoids,alkaloids,adenosine,etc.Among them,26 components were discovered from M.exotica for the first time.Conclusion This method can provide a basis for elucidating the pharmacological substance basis of root of M.ex-otica.
Keywords：Root of Murraya exoticaUPLC-Q-Orbitrap MSChemical compositionMass fragmentation patterns
Publication Date：2023-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 982-987,993 )