Determination of five fluorescent whitening agents in whitening masks by HPLC-FLD mehtod
DOU Yue
WANG Qianlei
TIAN Jinqiang
GUO Xing
PAN Keyi
Abstract：Objective To establish a method for the determination of five fluorescent whitening agents(FWA85,CBS351,FWA393,FWA378 and FWA368)in whitening masks by HPLC-FLD with fluorescence detector (HPLC-FLD).Methods The HPLC method was performed on an Agilent Zorbax Eclipse XDB-C18(4.6 mm×250 mm,5 μm) column by gradient elution using 20 mmol·L-1 ammonium acetate and methanol as mobile phase,at excitation wavelength of 365 nm and an emission wavelength of 430 nm,which can separate the target analytes successfully.Results The target analytes showed good linearity in the range of 0.05~30 μg·mL-1 with the correlation coefficients(r)greater than 0.99.The limits of detection(S/N=3)were 5.00~13.25 ng·g-1 and the limits of quantification (S/N=10) were 10.04~53.15 ng·g-1 for the target analytes.The feasibility of this method was demonstrated by the determination of FWAs in samples with spiked recoveries.The recoveries were in the range between 92.8% and 108.8%,and the relative standard deviations ranged from 1.8% to 7.3%.Conclusion This method was simple and had high recoveries for the determination of five fluorescent whitening agents in whitening masks.
Keywords：HPLCFluorescent whitening agentsWhitening masks
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 330-333,368 )