Determination of illegally added antidiabetic drugs in Tangjie Capsules by UPLC method
XIE Qiangsheng
ZHANG Zixuan
ZHANG Zhonghu
LI Qiyan
Abstract：Objective To develop an ultra performance liquid chromatography(UPLC) method for the simultaneous determination of illegally added antidiabetic drugs in Tangjie Capsules.Methods Agillent Zorbax SB-C18(2.1 mm×50 mm,1.8 μm)column was used with a mobile phase consisting of acetonitrile and 0.1% formic acid by gradient elution at a flow rate of 0.3 mL·min-1.The illegally added antidiabetic was detected by a photodiode array detector and a chromatogram with detection wavelength 235 nm was used.Results The linearity ranges of Phenformin Hydrochloride,Rosiglitazone,Glibenclamide were 6.1~122,5.15~103,5.35~107 μg·mL-1,respectively.The average precisions were 0.86%,1.03%,0.52% (n=6).The average recoveries were 97.1%,97.5%,97.0%,respectively,and the RSDs were 0.46%,1.04%,0.66%.Conclusion This method had the advantages of simple pre-treatment,fast detection,satisfactory recovery rate and good reproducibility.It was suitable for the determination of the antidiabetic agents and can be used as a reference in the detection of the illegally added chemical drugs.
Keywords：UPLCIllegally addedAntidiabetic drugs
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 200-202,214 )