Simultaneous determination of isorhamnetin-3-O-β-D-glucoside and formononetin in extract of leaves of Ilex cornuta by HPLC
NIE Liyun
CAO Xu
Abstract：Objective To establish a HPLC method for the simultaneous determination of isorhamnetin-3-O-β-D-glucoside and formononetin in extract of leaves of Ilex cornuta.Methods The HPLC analysis was carried out on Phenomenex C18 column(4.6 mm×250 mm,5 μm) with methanol-0.15% acetic acid solution as the mobile phase for gradient elution at a flow rate of 1 mL·min-1.The detection wavelength was 254 nm and the column temperature was 30 ℃.Results A good linear relationship were found in the the range of 6.97~278.8 μg·mL-1 for isorhamnetin-3-O-β-D-glucoside,2.96~118.4 μg·mL-1 for formononetin.Their average recoveries were 99.26% and 98.75%,respectively.Conclusion The method was accurate,reliable,and can be used for the quality control and evaluation of extract of leaves of Ilex cornuta.
Keywords：Extract of leaves of Ilex cornutaHPLCIsorhamnetin-3-O-β-D-glucosideFormononetin
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 142-144 )