Bioactive constituents studies on Gentianopsis paludosa against UC fibrosis based on the qRT-RNA method
LU Nianhua
ZHAO Huiqiao
LIU Xuefeng
JING Ming
CHEN Zhengjun
Abstract：Objective To study the bioactive constituents of Tibetan medicine Gentianopsis paludosa against UC fibro-sis.Methods Active fragment were obtained by silica gel and TLC from ethyl acetate active part which was extracted from Tibetan Gentianopsis paludosa.By method of qRT-PCR,collagen Ⅰ,collagen Ⅲ,α-SMA,and E-cad were detected to screen the active components after model rats being treated with active isolates.Results After purified and identified from ethyl acetate part using spectroscopy,four monomer compounds(1-hydroxy-3,7,8-Trimethoxyxanthones;1,8-Dihydroxy-3,7-Dimethoxyxanthones;1-hydroxy-3,7-Dimethoxyxanthones and 1,7-Dihydroxy-3,8-Dimethoxyxanthones)had sig-nificant bioactive on UC fibrosis.Conclusion The material basis of Gentianopsis paludosa against fibrosis concentrated in parts of ethyl acetate,and the active ingredient mainly owed to four xanthones.
Keywords：Active componentqRT-RNAGentianopsis paludosaUlcerative colitis
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 628-631,662 )