Determination of harpagoside and cinnamic acid in Yanhouqing Buccal Tablets by HPLC
LI Yisheng
LI Wenzhou
WEI Ping
QIU Shuqi
Abstract：Objective To establish a method for the determination of harpagoside and cinnamic acid in Yanhouqing Buccal Tablets.Methods Separations were carried out on a Waters Symmetry C18 column(4.6 mm×250 mm,5μm)by gra-dient elution with 0. 3% acetic acid and acetonitrile,the detection wavelength was 279 nm,the flow rate was 1. 0 mL·min-1 ,and the column temperature was 30 ℃. Results Chromatographic peaks of harpagoside and cinnamic acid were well separated with good precision and stability.Then the standard curve of harpagoside was rectilinear within the range of 0.039 65~0.396 5 μg(r=0.999 8),and the average recovery was 98.13 % with RSD of 1.20 %(n=6).The standard curve of cinnamic acid was rectilinear within the range of 0.099 68~0.996 8μg( r=0.999 7),and the average recovery was 98.87 % with RSD of 1.17%(n=6).Conclusion The method was accurate and reproducible,and can be used for the de-termination of cinnamic acid and harpagoside in Yanhouqing Buccal Tablets.
Keywords：Yanhouqing Buccal TabletsHarpagosideCinnamic acidDetermination
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 523-525 )