Study on qualitative and quantitative method for Wushengfulong Capsules
MOU Weiwei
LIU Yan
SUN Juan
Abstract:Objective To establish a qualitative and quantitative method of Wushengfulong Capsules. Methods Di-oscoreae rhizome were identified by microscope. Paeoniae radix rubra was identified by TLC. HPLC method was used to de-termine the effective content of paeoniflorin in Paeoniae radix rubra,The HPLC method was carried on C18 column using acetonitrile - 0. 1% phosphoric acid solution(14∶86)as mobile phase,and the detection wavelength was 230 nm,the flow rate was 1. 0 mL·min - 1 and the temperature of column was 30 ℃ . Results Dioscoreae rhizome,Paeoniae radix rubra can be identified by TLC. Paeoniflorin showed a good linear relationship in the range of 0. 060 2 ~ 1. 806 μg(r = 0. 999 5),the average recovery was 99. 47%(n = 9). Conclusion This method was simple,reliable,accurate,can be used to control the quality of Wushengfulong Capsules.
Keywords:Wushengfulong CapsulesPaeoniae radix rubraMicroscopic identificationTLCHPLC
Publication Date:2016-01-01
Online Publishing Date:2025-08-15(First online date of this platform, not the publication date of the document)
Pages:4( 396-398,407 )
