Determination of geniposide,hesperidin,honokiol,magnolol and tanshinone ⅡA in Ronggan Pills by HPLC
YUE Ning
HE Haiyan
JIANG Jun
Abstract：Objective To establish an HPLC method for the simultaneously determination of geniposide,hesperidin, honokiol,magnolol and tanshinone ⅡA in Ronggan Pills. Methods Geniposide,hesperidin,honokiol,magnolol and tanshi-none ⅡA were separated on Waters XTerra RP18(4. 6 mm × 250 mm,5 μm)column,detected at 238 nm and 40 ℃ . The mobile phase was acetonitrile - 0. 02% phosphate with gradient elution. The flow rate was 1. 0 mL·min - 1 . Results The linear ranges of geniposide,hesperidin,honokiol,magnolol and tanshinone ⅡA were 0. 027 4 ~ 0. 823 2,0. 055 9 ~ 1. 677 0, 0. 008 3 ~ 0. 249 0,0. 024 3 ~ 0. 730 2,0. 002 6 ~ 0. 079 5 μg and the average recovery were 99. 01%(1. 35% ),98. 95%(1. 54% ),98. 28%(1. 49% ),100. 93%(2. 08% ),98. 91%(1. 84% ),respectively. Conclusion The method was con-venient,quick,accurate and reproducible,it can be used for the quality control of Ronggan Pills.
Keywords：Ronggan PillsGeniposideHesperidinHonokiolMagnololTanshinone ⅡADeterminationHPLC
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 390-392,395 )