Simultaneous determination of sodium acetate and sodium citrate in Intraocular Perfusion Liquid by HPLC
LI Yonggui
LENG Jiawei
ZHANG Xixia
WANG Jing
Abstract：Objective To establish an HPLC method for the simultaneous determination of sodium acetate and sodium citrate in Intraocular Perfusion Liquid.Methods This method was adopted with Cosmosil 5C1 8 -PAQ(4.6 mm ×250 mm) column,the mobile phase was consisted of hosphoric acid aqueous solution (water with phosphoric acid to adjust the pH to 2.1 5) -methanol (95∶5).The detection wavelength was 21 0 nm,the column temperature was 30 ℃,the flow rate was 1 .0 mL·min -1 .Results The linear range of sodium acetate was 0.1 41 1 ~0.329 2 mg·mL -1 ,the average recover was 1 00.71 % and the RSD was 0.57%;The linear range of sodium citrate was 0.089 5 ~0.208 9 mg·mL -1 ,the average re-cover was 1 00.63% and the RSD was 0.54%.Conclusion The method was simple,rapid,accurate,reliable and specific.
Keywords：HPLCIntraocular Perfusion LiquidSodium acetateSodium citrateDetermination
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 331-333 )