Simultaneous determination of chlorogenic acid,caffeic acid,puerarin,daidzin in Xiaoer Jiebiao Granules by HPLC
HE Haiyan
ZHANG Ye
LIU Hongming
Abstract：Objective To simultaneous determine the contents of chlorogenic acid,caffeic acid,puerarin,daidzin in Xi-aoer Jiebiao Granules by HPLC. Methods Agilent Zorbax XDB - C18 column(4. 6 mm × 250 mm,5 μm)was adopted with acetonitrile - 0. 1% phosphoric acid as the mobile phases by gradient elution. The column temperature was 40 ℃ . The de-tection wavelength was changed. Results The linear ranges of chlorogenic acid,caffeic acid,puerarin,daidzin were 0. 051 46 ~ 1. 029 2 μg,0. 024 70 ~ 0. 494 0 μg,0. 050 57 ~ 1. 011 4 μg,0. 008 292 ~ 0. 165 8 μg,respectively. The aver-age recoveries were between 98. 77% ~ 99. 98% with RSDs less than 2. 00% . Conclusion The method was accurate,sen-sitive,reproducible,it can be used for the overall assessment of the quality of Xiaoer Jiebiao Granules.
Keywords：Xiaoer Jiebiao GranulesHPLCChlorogenic acidCaffeic acidPuerarinDaidzin
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 214-216 )