Determination of sarsasapogenin in Runing Pill by HPLC
XIAO Bing
WU Yanhong
ZHU Weifeng
XU Yan
Abstract：Objective To establish an HPLC method for the determination of sarsasapogenin in Runing Pill. Methods The Waters SunFire C18 column(4. 6 mm × 250 mm,5 μm)was used with the mobile phase of methanol - water (90∶10). The flow rate was 1. 0 mL·min - 1 ,the evaporative light scattering detector was adopted for the detection whose drift tube temperature was 55 ℃ and the gas pressure was 350 kPa. Results The sample size of sarsasapogenin in the range of 1. 18 ~ 5. 90 μg showed a good logarithmic linear relationship with the integral value of the peak area( r =0. 999 5). The average recovery was 97. 46% ,the RSD was 1. 37%(n = 9). Conclusion The method was accurate and specific which can be used for the quality control of Runing Pill.
Keywords：Runing PillSarsasapogeninHPLCDetermination
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 212-213,229 )