Inspection of saponins from Lonicerae flos in Kanggan Granules by HPLC - ELSD
LIU Qingzhi
WANG Liguo
CUI Weiliang
LIN Yongqiang
GUO Dongxiao
Abstract：Objective To establish an HPLC - ELSD method for the inspection of Shanyinhua( Lonicerae flos)in Kanggan Granules. Methods Macranthoidin B and dipsacoside B,which are both characteristic triterpenoid saponins of Lonicerae flos,were used as the detection targets. The method was carried out on a Thermo Hypersil Gold C18 column(4. 6 mm × 250 mm,5 μm)with the mobile phase consisted of water - acetonitrile by gradient elution. The column temperature was 32 ℃,and the flow rate was 1. 0 mL·min - 1 . The drift tube and atomization temperatures of ELSD were set at 90 ℃and 60 ℃,respectively,with the flow rate of N2 at 1. 6 L·min - 1 . Results 53 batches of samples were measured. At least one characteristic triterpenoid saponin of Lonicerae flos was detected in 49 batches of samples,which indicated Lonicerae flos had been used in corresponding batches of Kanggan Granules. Conclusion The established method was highly accurate, specific,and reliable,which can be applied to detect the use of Lonicerae flos in Kanggan Granules.
Keywords：Kanggan GranulesLonicerae japonicae flosLonicerae flosMacranthoidin BDipsacoside B
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 205-208 )