Growth inhibition and apoptosis in human colorectal cancer cells induced by 5F - extracted from Pteris semipinnata L.
GAO Xiaosheng
LYU Yingnian
WU Kefeng
LIU Yi
FU Weiyu
CHEN Ruotai
LI Li
Abstract：Objective To examine the antiproliferative and proapoptotic effects of 5F(ent - 11α - hydroxy - 15 - oxo- kaur - 16 - en - 19 - oic - acid),a compound isolated from Pteris semipinnata L.(PsL),on HCT - 116 colorectal cancer (CRC)cells. Methods Cytotoxicity was examined using a MTT method. Cell apoptosis was determined through expression of representative apoptosis - related proteins and Annexin V/ PI staining. Reactive oxygen species(ROS)level was meas-ured using standard protocols. Results 5F inhibited the proliferation of HCT - 116 cells in a concentration - and time - de-pendent manner. 5F increased the ratio of Bax/ Bcl - 2 in HCT - 116 cells was identified by western blotting analysis. 5F could enhance both the inhibitory effect of cisplatin on growth of HCT - 116 cells and the level of apoptosis induced by cis-platin in HCT - 116 cells. 5F did not increase ROS,but reduced ROS production induced by cisplatin. Conclusion 5F can induce CRC cell apoptosis and therefore represents therapeutic potential for cancer treatment.
Keywords：Pteris semipinnata L.Colorectal cancerApoptosisReactive oxygen species
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 193-196,211 )