The effect of corilagin for IAPP,GLP -1 and INS of rats which fed by fructose

ZHANG Jian-bo; CHEN Ping; XIE Yu; GONG Jian-yu; FAN Yuan

Abstract：Objective To feed male Wistar rats with fructose solution,and inject different concentration of corilagin so-lution to Wistar rats,determine the changes of the concentration of IAPP,GLP - 1)and INS in rats serum. Methods To give same concentration of fructose solution to Wistar rats three weeks by intragastric administration. In the fourth week,dif-ferent concentration of corilagin solution was admined by intraperitoneal injection,once every two days and three times. In the seventh day,blood serum of rats was collected and the concentrations of IAPP,GLP - 1 and INS were determined by ELISA. Results After fed fructose solution,the rats secreted insulin was decreased,the concentration of IAPP was reduced and the concentration of GLP - 1 was increased;After injected the concentration of 20 mg·kg - 1 corilagin,the insulin secre-tion was increased,the concentration of IAPP was decreased significantly,the concentration of GLP - 1 was increased signifi-cantly;When injected the concentration of 40 mg·kg - 1 corilagin,the insulin secretion was decreased significantly to com-pared with control group,the concentration of IAPP was increased significantly and higher than control group,the concentra-tion of GLP - 1 was reduced significantly and lower than control group. Conclusion Corilagin at low concentration can pro-mote the generation of GLP - 1,stimulate insulin secretion and inhibit the formation of IAPP. The experiment provided some basis on the further research of corilagin for the hormonal changes related to insulin resistance in rats model.