Study on the risk assessment of drug hepatoxicty by high content analysis
GAO Yang
HU Yu-chi
ZUO Ze-ping
WANG Zhi-bin
JIN Jia-jin
WANG Li-na
CHENG Yuan
Abstract：Objective To study a standard method for the determination of drug hepatoxicty evaluation index using high content analysis. Methods To investigate the cytotoxic effect of four drugs on HepG2 cells in human hepatocellular carcinoma cells by using ToxInsight Drug Induced Liver Injury Assay Cartridge and combining high content live cell imaging analysis platform. Through the comprehensive analysis of 5 indexes,the number of cells,DNA content,GSH level,ROS level and mitochondrial membrane potential,the liver toxicity of the tested samples were evaluated. Results The results of fluox-etine and rosiglitazone were negative. The results of mefenamic acid and ticlopidine were positive,which indicated that the risk of liver toxicity was high,and the risk of liver toxicity of mefenamic acid was higher than that of ticlopidine. Conclusion HCA was based on living cells,multi parameters,real - time and high content,can achieve a variety of biological activity, drug toxicity of the early and rapid detection. It was sensitive,stable,simple and feasible. HCA can be used to evaluate the risk of drug hepatoxicty in vitro.
Keywords：High content analysisDrug induced liver injuryAssessment of toxicity
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 688-691,702 )