Improvement of assay method of metformin hydrochloride in Metformin and Glipizide Tablets
WANG Chang-dan
ZANG Heng-chang
ZUO Yan-hong
Abstract：Objective To establish an HPLC method for the determination of metformin hydrochloride in Metformin and Glipizide Tablets. Methods The separation was performed on C18 column(4. 6 mm × 150 mm,5 μm),and the mobile phase was composed of 0. 05% sodium heptane - 1 - sulphonate solution(with 10% phosphoric acid solution to adjust pH value to 4. 0)- acetonitrile(84:16)with the flow rate of 1. 0 mL·min - 1 . The detection wavelength was 233 nm. The col-umn temperature was 30 ℃ . Results The linear range of metformin hydrochloride was 10. 02 ~ 30. 06 μg·mL - 1(r =0. 999 9). The average recovery rate was 101. 0% with RSD of 0. 66%(n = 9). Conclusion The established method was better and more accurate than the original national registered standard specificity.
Keywords：HPLCMetformin and Glipizide TabletsMetformin hydrochloride
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 574-576,610 )