Simultaneous determination of five components in Chaiyin Oral Liquid by HPLC
ZHU Ri-ran
Abstract：Objective To establish an HPLC method for the simultaneous determination of five components in Chaiyin Oral Liquid. Methods Using Zorbax SB - C18 column(4. 6 mm × 250 mm,5 μm),acetonitrile - 0. 1% phosphoric acid solution as mobile phase. The flow rate was 1 mL·min - 1 ,the detection wavelength was at 210 nm. Results The linear ranges of chlorogenic acid,amygdalin,puerarin,baicalinand,phillyrin were 0. 388 ~ 9. 70 μg(r = 0. 999 9),0. 736 ~ 18. 40μg(r = 0. 999 8),0. 21 ~ 5. 25 μg(r = 0. 999 8),0. 242 ~ 10. 6 μg(r = 0. 999 9),0. 868 ~ 21. 7 μg(r = 0. 999 9),respec-tively. The average recoveries( n = 6)were 96. 80%( RSD = 0. 29% ),103. 50%( RSD = 1. 29% ),103. 60%( RSD =1. 12% ),102. 90%(RSD = 0. 88% ),100. 90%(RSD = 0. 90% ),respectively. Conclusion The results indicated that the method was simple,accurate,highly selective and reproducible,thus it can be used as quality control for Chaiyin Oral Liquid.
Keywords：Chaiyin Oral LiquidChlorogenic acidAmygdalinPuerarinBaicalinPhillyrin
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 509-511 )