Effects and mechanisms of resveratrol on proliferation in human colonic adenocarcinoma cell line HT -29
WANG Jin-ying
QIN Mi-mi
HAN Ying-ying
ZHANG Yan
CAO Zhang
Abstract：Objective To evaluate the effect and mechanism of resveratrol inhibiting proliferation and inducing apopto-sis on human colon cancer cells HT - 29. Methods The proliferation of human colon cancer line HT - 29 was measured by CCK - 8 assay. Cell cycle and apoptosis were measured using Flow cytometer(FCM). Western blotting was used to detect PDCD4 protein expression. Results Different concentrations of resveratrol on cell growth was inhibited in a dose - depend-ent manner. The effect on cycle distribution and appotosis were significance difference(P < 0. 05). There was significant difference of the expression of PDCD4 on HT - 29 cells before it treated by resveratrol and after that(P < 0. 01). Conclu-sion Resveratrol could inhibite the proliferation,and induce cell cycle arrest and appotosis of HT - 29 cell line,which may be related to overexpression of PDCD4 on HT - 29 cells.
Keywords：ResveratrolHT - 29Cell proliferationPDCD4
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 439-441,446 )