Study on activation microglia′s α7 - nAChR for reducing neuron toxicity induced by Aβ protein

LEI Lu-wen; LI Wei-xuan; FENG Zhi-ming; DENG Chong; CHENG Xiang

Abstract：Objective To investigate the activation of microglia′s α7 neuromal acetylcholine receptor(α7 - nAChR) for reducing neuron toxicity,which resulted from accumultation of β - amyloid peptide 1 - 42(Aβ1 - 42 )increased levels of pro - inflammatory factors,contributed to the local jnflammatory response. Methods The cortex of fetation 18 days rats were isolated and minced. The growth of neurons was observed with contrast phase miroscop. The cells were identified by im-munocytochemistry after immunostaining with MAP - 2 and NSE on the tenth day. The tenth day neurons coculture with mi-croglai cells in the transwells. The cells randomly divided into four groups:blank control,Aβ protein group,coculture group;nicotin pretreat group. Neurons apoptosis model was established by added Aβ1 - 42 protein in each group after 96 hours,and the apoptosis rats was analysed by FCM and fluorescence microscopy. Results Neuron cells after 10 days incubation were identified by immunocytochemistry,immunofluorescence staining and DAB staining show neuron cells stainning positive,pu-rity ratio reached 94. 49%. The neuron apoptosis ratio in activative microglia′s α7 - nAChR group was lower than that in in-activative group. Conclusion Activative microglia′s α7 - nAChR reduced neuron apoptosis ratio,so that protected neuron from the toxic effect induced by pro - inflammatory factors.