Determination of paeoniflorin and berberine hydrochloride in Baidai Pills by HPLC
MA Shuai
GE Jie-ying
ZHOU Peng
Abstract：Objective To establish an HPLC method for the determination of paeoniflorin and berberine hydrochloride in Baidai Pills. Methods Paeoniflorin and berberine hydrochloride were separated on Wondasil C18 Superb(4. 6 mm × 250 mm,5 μm)column. The mobile phase was acetonitrile and 0. 3% phosphoric acid - solution in gradient mode. The flow rate was 1. 0 mL·min - 1 . The detection wavelength for paeoniflorin was set at 230 nm,and 320 nm for berberine hydrochlo-ride. Results The linear range of paeoniflorin and berberine hydrochloride were 0. 106 29 ~ 2. 125 8 μg·mL -1(r = 0. 999 9)and 0. 060 21 ~ 1. 204 2 μg·mL - 1(r = 0. 999 9),and their average recoveries were 98. 45% and 100. 52% respective-ly. Conclusion The method can be used for the quality control of Baidai Pills.
Keywords：Baidai PillsPaeoniflorinBerberine HydrochlorideHPLC
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 398-400 )