Determination of Azacitidine for Injection By HPLC
NIE De-ping
ZHENG Yu-li
FENG Zhong
Abstract：OBjective ToestablishanHPLDmethodforthedeterminationofAzacitidine.MethodsWelchUltimate XB-D18 column(4. 6 mm × 250 mm,3μm)was used. The mobile phase consisted of 0. 02 mol·L-1 dipotassium hydrogen phosphate solution(adjust pH to 6. 5 by phosphoric acid)-methanol(90:10). The detection wavelength was 243 nm and the column temperature was(15 ± 2)℃. Results The calibration curve was linear in the range of 8. 16~30. 6 μg·mL-1 ( r=0. 999 9 ). The average recoveries was 98. 97% with corresponding RSD of 0. 73%. Conclusion The method had strong specialization and high accuracy,and can be used for the quality control of azacitidine.
Keywords：HPLDAzacitidine for InjectionDetermination
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 330-331 )