Determination of anisodamine,scopolamine and atropine in Huashanshen by HPLC
CHEN Yu
YAN Xiao-nan
YANG Xiao-hong
Abstract：Objective To establish an HPLC method to determine anisodamine,scopolamine and atropine in Huashan-shen. Methods Anisodamine,scopolamine and atropine were assayed on an Agilent Zorbax SB-Aq C18( 4. 6 mm × 250 mm,5 μm)column;The mobile phase composed of acetonitrile-50 mmol·L-1 potassium dihydrogen phosphate( contai-ning 0. 08% triethylamine,adjust pH to 6. 0 with phosphatic acid)(15:85)at a flow rate of 1. 0 mL·min-1;The determi-nation wavelength was 210 nm. Results The calibration curves were linear in the range of 0. 058 8 ~2. 949 1 μg( r=0. 999 9)for anisodamine,0. 145 7~7. 284 1 μg( r=0. 999 9)for scopolamine and 0. 161 7~8. 084 4 μg( r=0. 999 9)for atropine,respectively. The average recoveries( n=6 )of anisodamine,scopolamine and atropine were 100. 03%( RSD=0. 13%),100. 77%( RSD=0. 97%)and 99. 20%( RSD =0. 80%),respectively. Conclusion The method was simple and reliable,accurate,repeatable and can be used for the quality control of Huashanshen.
Keywords：HuashanshenAnisodamineScopolamineAtropineHPLC
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 209-211,214 )