Establishment of a rat model of atherosclerosis

XING Yu-gui; MA Fei; SUN Hong-sheng

Abstract：Objective To establish the model of atherosclerosis with easy operation,obvious effect in rats. Methods The 8 weeks old Wistar rats were divided into the high fat diet group and the basic diet group. The high diet rats were feed with high fat food which was prepared accooding to a certain proportion and Vitamin D3 by gavage. The basic diet rats were given ordinary rat food and physiological saline by gavage. After they were feed 8 weeks,biochemical parameters such as blood glucose and blood lipid and pathological changes were observed,the expression of MMP-9 was observede by immunohisto-chemical method. Results After the rats were feed with high diet food 8 weeks,the weight,the blood glucose,triglyceride,to-tal cholesterol,low density lipoprotein and other biochemical parameters of the high diet rats,especially triglyceride and the to-tal cholesterol,were higher than the basic diet rats'. There was a lot of calcified plaque was found in the high fat diet rats'ca-rotid artery,the expression of MMP-9 in the high fat diet rats increase,but there was no significant change in the basic diet rats. Conclusion The rats were feed with high fat food which include 7% lard oil,5% cholesterol,3%white sugar,0. 5% so-dium cholate,0. 2% propyl sulphur pyrimidine,84. 3% ordinary rat food,and 700 000 U·kg-1 Vitamin D3 by gavage. After 8 weeks,ideal rat model for the study of atherosclerosis can be established successfully.