Study on extraction process of sesquiterpene lactones from Flos Inulae and the anti-cancer effect in vitro
HUANG Yi-fan
WANG Shuai
MENG Xian-sheng
BAO Yong-rui
Abstract：objective To inspect the influencing factors of extracting sesquiterpene lactones from Flos Inulae,and op-timize the extraction process by orthogonal test. To investigate the pharmacological activity of anti-cancer effect combining with the experiments of antineoplastic pharmacodynamics in vitro,and provide references and theoretical basis for the devel-opment and utilization of Flos Inulae. Methods Four main factors of ethanol concentration,extraction time,times and etha-nol amount were investigated to screen the optimum extraction process with ABL as investigating index by orthogonal experi-mental design. This experiment used MTT assay to evaluate the antitumor activities of sesquiterpene lactones to the cell line of HepG2 cells,and to explore the rule of the antitumor activities. Results The optimum conditions for the extraction of Flos Inulae were:15 times amount of 80% ethanol,3 times reflux extraction,2 hour each time. The sesquiterpene lactones suppressed the proliferation of HepG2 cells,it had a good does-time-effect relations. Conclusion The experiment was designed to optimize the extraction process of Flos Inulae and the antitumor activities of sesquiterpene lactones in vitro,it provided the basis of experiment and theoretical basis for Flos Inulae.
Keywords：Flos InulaeSesquiterpene lactonesorthogonal experimental designExtraction processAntitumor activi-ties
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 69-71 )