Determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Biyan Syrup by HPLC
RAN Ya-dong
YUAN Huan-huan
GUAN Liu
Abstract：Objective To establish an HPLC method for the determination of ephedrine hydrochloride and pseudo-ephedrine hydrochloride in Biyan Syrup. Methods The Agilent Zorbax SB-C18(4. 6 mm × 250 mm,5 μm)column was used. The mobile phase consisted of acetonitrile-0. 20% phosphoric acid(containing 0. 2% triethylamine)(2:98)with the flow rate of 1. 0 mL·min-1 . The detection wavelength was 206 nm and the column temperature was 25 ℃. Results The linear range of ephedrine hydrochloride and pseudoephedrine hydrochloride was 0. 124~1. 86μg(r=0. 999 9)and 0. 103 8~1. 557 μg(r=0. 999 9)with the average recovery of 99. 99%(RSD=1. 24%,n=6)and 99. 33%(RSD=1. 93%,n=6),respectively. Conclusion The method was accurate and reproducible. It can be used for the determination of ephed-rine hydrochloride and pseudoephedrine hydrochloride in Biyan Syrup.
Keywords：Biyan SyrupEphedrine hydrochloridePseudoephedrine hydrochlorideHPLC
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 699-701 )