SimultaNeous determiNatioN of gallic acid,metHyl gallate aNd etHyl gallate from gallNut extracts by HPLC
SHEN Hua
WANG Zheng
LI Lei
LI Hong-yan
Abstract：Objective To establish a method for the simultaneous determination of gallic acid,methyl gallate and ethyl gallate from gallnut extracts. MetHods HPLC was used to quantitative analysis. The Waters Symmetry C18(4. 6 mm × 250 mm,5 μm)was used,and mobile phase was composed of methanol-water(25:75)(adjust pH to 4. 5 with acetic acid). The detection wavelength was 273 nm at 30℃. The flow rate was 1. 0 mL·min-1 . Results The gallic acid,methyl gallate and ethyl gallate's spotting recovery were 99. 79%,99. 60%,99. 45%,and the linear ranges were 0. 2~3. 2 μg,0. 002~0. 16 μg,0. 010 8~0. 54 μg,respectively. CoNclusioN The results were accurate and the reproducibility was good. The method can be used for the quality control of the product.
Keywords：Gallnut extractsGallic acidMethyl gallateEthyl gallateDeterminationHPLC
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 631-632,650 )