INHibitioN effects of bezafibrate iN coordiNatioN witH cisplatiN oN cell growtH aNd iNduciNg apoptosis of luNg adeNocarciNoma cells
WANG Gui-ping
ZHANG Yan-tao
LIU Xin-yan
PAN Xue-bing
ZHOU Qiong
GUO Li-lin
Abstract：Objective To observe the effects of peroxisome proliferator-activated receptorα( PPARα)agonist bezaf-ibrate combinated with cisplatin( DDP)on proliferation and apoptosis of lung adenocarcinoma( AC)A549 cell and the pos-sible mechanism. MetHods CCK8 kit assay was used to detect growth inhibiton curve of A549 cells treated with various concentrations of bezafibrate. The median-effect method was used to evaluate the combination effect. Cell apoptosis was e-valuated with flow cytometry,and the expression of VEGF and HIF-1αmRNA were analyzed using real-time PCR( qRT-PCR). Results Bezafibrate combinated with DDP showed significately synergy inhibition effect,and the IC50 was 15. 92μmol·L-1 when the two agents were combined. The combination index(CI)was less than 1 when the concentration of bez-afibrate<588 μmol·L-1 and cisplatin<206 μmol·L-1 ,namely two drugs combination showed synergy effect. The cell apoptosis rate of A549 cells was significately higher than single drug group(P<0. 05). The expression level of VEGF and HIF-1α mRNA were significately lower in combination group than in bezafibrate or cisplatin group(P<0. 05). CoNclu-sioN Our results demonstrated that bezafibrate combinated with cisplatin showed synergy inhibition effect on lung AC, which may be related with the down-expression of VEGF and HIF-1α.
Keywords：BezafibrateCisplatinLung adenocarcinomaSynergy effectPPAR
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 621-625 )