Study on identification of Compound Sanhuang Tincture by TLC and determination of ethanol
DING Chang-ling
ZHAO Yong-de
GAO Hua
CUI Jun-feng
Abstract：Objective To establish an advanced TLC identification method of Compound Sanhuang Tincture,and a method for the determination of ethanol. Methods The Rhizoma coptidis,Cortex phellodendri and Synthetic borneol were i-dentified with mobile phase of microemulsion form incacid by silica gel G thin layer plate. The consis of microem ulsion was toluene:toluene - isopropanol - ethyl acetate - methanol - H2 O = 6:1. 5:3:1. 5:0. 3,chloroform - methanol - H2 O(30:15:4),benzene - ethyl acetate(19:1). The temperature was 26 ℃,and the relative humidity 62% . The determination of ethanol was carried out with Agilent 6890N gas liquid chromatography. Results The spots in the chromatogram of the test solution corresponding to the position of each reference substance had the same color. The content of alcohol was 48. 0% ~ 50. 0% . Conclusion The method was simple and can he used for the thin layer identification of Compound Sanhuang Tincture and the determination of ethanol.
Keywords：Compound Sanhuang TinctureTLCEthanol content
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 585-588 )