Influence of colon carcinoma microenvironment on cell morphology,proliferation and expressions of CD13 and CD133 of bone marrow mesenchymal stem cells
JING Ming
CHEN Zheng-jun
WANG Ya-li
ZHANG Yan-xia
LIU Xue-feng
Abstract：Objective To investigate the effect of Colon carcinoma microenvironment on cell morphology,proliferation and expressions of CD13 and CD133 of bone marrow mesenchymal stem cells. Methods Control group was bone marrow de-rived stroma cells cultured in DMEM medium,experimental group was(BMSCs)SW480 cells and bone marrow derived stro-ma cell(BMSCs)co - cultured in transwell chamber. The cell morphology of BMSCs was observed by microscope,and the generation of BMSCs were detected by MTT method. Cell cycle and expressions of CD13 and CD133 were analyzed by flow cy-tometry(FCM). Results Compared with control group,BMSCs in experimental group had the feature of malignant tumor cells,the generation speed significantly quicker and the percentage of S phase was significantly more than control group,The expressions of CD13 in experimental group(89. 7%± 9. 5% )were significantly higher than control group(67. 1%± 8. 1% ) (P ﹤ 0. 01),and the expressions of CD133 in experimental group(90. 5% ± 6. 4% )were significantly higher than control group(4. 3%± 1. 2% )(P ﹤ 0. 01). Conclusion These findings suggested that transwell chamber can help to achieve co -cultivation of SW480 cells and BMSCs and induce malignant transformation of BMSCs cell,the mechanism could be related to the changes of bionomics such as cell morphology,reproductive activity and cell surface marker.
Keywords：Colon carcinoma microenvironmentBMSCsCell cycleCD13 and CD133
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 497-500,510 )