Determination of cyanide in caffeine by isonicotinic acid-pyrazolone colorimetric method
DU Chuan-qiang
NIE Lei
Abstract：Objective Using isonicotinic acid-Pyrazolone colorimetric method for the content determination of CN- in Caffeine. Methods In neutral medium,the cyanide reacted with chloramine T to form cyanogen chloride. Cyanogen chlo-ride reacted with isonicotinic acid and then hydrolyzed to form glutaconaldehyde which reacted with Pyrazolone to form blue dye. The cyanide content had Positive correlation with absorbance of the blue dye at 638 nm. Results The range of linear relationshiP of cyanide was 0. 007 74~0. 193 44 μg·mL-1(r=0. 999 97). The average recovery was 100. 2%(n=9);The relative standard deviation was 0. 49%. Conclusion The method was sensitive and accurate,which was suitable for the determination of cyanide in caffeine.
Keywords：Isonicotinic acid-Pyrazolone colorimetric methodCaffeineCyanide
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 454-456 )