Micronucleus test of the right - handed rabeprazole sodium in mice

ZHANG Hai-ting; QI Xia

Abstract：Objective To study mouse micronucleus test of rabeprazole sodium administered orally,evaluation dextral rabeprazole sodium on chromosome damage in animals to predict their genetic toxicity. Methods Healthy male BALB / c mice were 60,were randomly divided into low,medium and high dose groups,vehicle control group and positive control group,n = 6. Intragastric administration of rabeprazole sodium were handed 125,250,500 mg·kg - 1 ,and vehicle control group was given sterile distilled water,dosing volume 40 mL·kg - 1;Positive control group was given intraperitoneal injec-tion of cyclophosphamide amide 50 mg·kg - 1 ,dose volume of 20 mL·kg - 1 . After 24 h and 48 h of administration the ani-mals were killed by cervical dislocation,took sternum bone marrow conventional producers,Giemsa staining. Microscopy double - blind read the piece,each mouse was observed 2 000 polychromatic erythrocytes(PCE),micronuclei count ap-peared polychromatic erythrocytes,calculated micronucleus rate,micronucleus rate expressed in parts per thousand,and cal-culated the total proportion of red blood cells and red cells. Results 24 h after oral administration,the bone marrow micro-nucleus incidence of low,medium,high - dose group were 1. 92‰,1. 58‰,1. 83‰,respectively,compared with the vehicle control group(1. 75‰),there had no significant differences. But there were very significant differences(P ﹤ 0. 01)between it and positive control group(23. 92‰). After gavage for 48 h,the incidence of bone marrow of low,medium and high dose group were 1. 75‰,1. 58‰,1. 50‰,respectively,and vehicle control group(1. 83‰)had no significant difference,but had a very significant difference(P ﹤ 0. 01)compared with positive control group(21. 83‰). Conclusion Under the ex-perimental conditions,the result of micronucleus test of the right - handed rabeprazole sodium administration orally in mice was negative.