Determination of Afatinib Tablets by HPLC
HAN Zhong-li
HAO Gui-zhou
CHEN Xiao-wei
Abstract：Objective To establish an HPLC method for the determination of Afatinib Tablets. Methods A stable HPLC method was established,and the chromatography was accomplished on Phenomenex Luna C18 column(4. 6 mm × 250 mm,5 μm),the mobile phase consisted of 0. 02 mol·L-1 of potassium dihydrogen phosphate( adjusted to pH 6. 0 with 2 mol·L-1 sodium hydroxide solution)-acetonitrile( 50:50 ),the column temperature was 30 ℃,the detection wavelength was 252 nm and the flow rate was 1. 0 mL·min-1. Results The linear range of afatinib was 20~120 μg·mL-1(r=0. 999 9),the average recovery was 100. 3% with corresponding RSD of 0. 93%. Conclusion The method was specific,ac-curate,convenient and rapid.
Keywords：HPLCAfatinib TabletsContent
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 337-338 )