Experimental observation on the analgesic effect of NT on frog rectus abdominis N2 -receptor
GAO Hong-jin
XU Yun-lu
WANG Shao-ming
CHEN Yan
Abstract：Objective To study the analgesic effect and the drug-receptor interactions with frog rectus abdominis N2-receptor of the neurotoxin from Naja atra venom( NT). Methods Three model of pain( tail electric stimulation-vocali-zation test ,the hot-plate test ,and writhing responses in mice)were used. The effects of NT on acetylcholine-induced i-solated frog rectus abdoninis were observed. Results NT could dose-dependently raise the pain threshold in hot-plate test in mice and inhibit the writhing reaction induce by acetic acid in mice . The analgesic effect of NT effect at 2 h and peaked at 5 h. NT produced a concentration-dependent parallel shift to the right of dose-response curve of acetylcholine applied exogenously with no change in the maximal asymptotic response. Conclusion NT had a better analgesic effect on the pain due to hot- plate ,acetic acid and electric stimulation. NT was determined as the competitive antagonists of N2 -AChR.
Keywords：Naja atraNeurotoxinAnalgesicCompetitive antagonist
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 315-318 )