Determination of glucose in neutral peritoneal sialysis solution(lactate)by HpLC
TENG Xia
TANG Su-hua
Abstract：Objective To establish an HPLC method for the determination of glucose in neutral peritoneal sialysis so-lution(lactate). Methods Waters Sugar Park I column(6. 5 mm × 300 mm,6. 5 μm),the mobile phase was water with the flow rate of 0. 5 mL·min - 1. The column temperature was 80 ℃. The set temperature of differential detector was 40 ℃ and the sample size was 20 μL. Results The linear range of glucose was 1. 133 78 ~ 6. 802 68 mg·mL - 1(r = 1. 000 0). The average recovery of containing 1. 5% glucose in neutral peritoneal sialysis solution(lactate)was 99. 1% ,RSD = 0. 4 %(n =9);The average recovery of containing 2. 5% glucose in neutral peritoneal sialysis solution(lactate)was 100. 1% ,RSD =0. 2%( n = 9);The average recovery of containing 4. 25% glucose in neutral peritoneal sialysis solution( lactate)was 100. 2% ,RSD = 0. 3%(n = 9). Conclusion The method was simple,rapid,accurate and can be used for neutral perito-neal sialysis solution(lactate)for quality control .
Keywords：HPLCNeutral peritoneal sialysis solution(lactate)GlucoseDetermination
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 274-275,281 )