ERK1/2 signal pathWay mediated propofol protected on cultured rat cardiomyocytes damaged by H2 O2
LIANG Yan-li
TANG Min
XU Cong-min
Abstract：Objective To investigate the influence and mechanism of propofol on cardiomyocytes damaged by H2 O2 . Methods Cardiomyocytes from the hearts of 1 ~ 3 - day - old neonatal rats were prepared by a modified method. Treated cultured rat cardiomyocytes by H2 O2 ,propofol(12. 5,25,50 μmol·L - 1 ). MTT was used to detect cell viability. Malondial-dehyde(MDA)was determined by measuring thiobarbituric acid - reactive substances. Superoxide dismutase(SOD)was assayed by xanthine oxidase method. The rates of apoptosis of cardiomyocyte were detected by flow cytometry. The expression of ERK1 / 2,Bcl - 2 and Bax were tested by western blotting. Results Propofol suppressed the cardiomyocyte death induce by H2 O2(P < 0. 01),and decreased the production of MDA(P < 0. 01),promoted the ability of SOD(P < 0. 01). The ap-optosis rates of propofol group significant different from that of H2 O2 group(P < 0. 05). Propofol activated the phosphorated ERK1 / 2. The expression of Bcl - 2 was increased while that of Bax was suppressed. Conclusion Propofol protected on cul-tured rat cardiomyocytes damaged by H2 O2 via ERK1 / 2 signal pathway.
Keywords：PropofolCardiomyocytesERK1/2H2O2
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 257-260 )