Multi-enzyme cascade silk fibroin hydrogel for promoting diabetic wound healing
ZHANG Jiayao
HU Fan
Abstract：To address the complex microenvironment of diabetic wounds,a multi-enzyme cascade system composed of glucose oxi-dase and horseradish peroxidase(GOX-HRP)was incorporated into hyaluronic acid-modified silk fibroin(SF)hydrogel to prepare hy-drogel dressings capable of promoting wound healing in diabetes.Through examinations of the mechanical properties,catalytic perform-ance and cytotoxicity of the hydrogels,as well as animal experiments,the therapeutic efficacy of this hydrogel on diabetic wounds was investigated.The results demonstrated that the adhesion strength of the hydrogel tissue reached 29.9 kPa,which was 4.75 times higher than the unmodified hydrogels,and the elastic modulus was 14.8 kPa.In terms of effectively generating oxygen,reducing glucose levels and scavenging reactive oxygen species,the duration of action all reached 24 h(those performance absent in the enzyme-free hydro-gels).The cell survival rate reached 95%.The wound healing rate of mice reached 81%within two weeks,which was 12%higher than that of the control group.The multi-enzyme cascade SF hydrogel can effectively facilitate the healing of diabetic wounds,exhibits no cytotoxicity and broad prospects for clinical applications.
Keywords：Silk fibroinMulti-enzyme cascadeHydrogelsDiabetic woundsWound repair
Publication Date：2026-04-30
Online Publishing Date：2026-09-11(First online date of this platform, not the publication date of the document)
Pages：6( 133-138 )