Study on transdermal permeation characteristics of full molecular weight sodium hyaluronate
WANG Zheng
FAN Yumei
LUO Xiaolan
LING Peixue
Abstract：To systematically evaluate the transdermal penetration behavior and effects of full molecular weight sodium hyaluronate(FMW-SH),we took FMW-SH as the research object and determined the molecular weight distribution of FMW-SH by high perform-ance gel chromatography.Using a 3D skin model,we evaluated its transdermal penetration efficiency.Multiple analytical approaches were employed to assess FMW-SH's biological effects,including ELISA for typeⅠcollagen synthesis,scratch assay for keratinocyte mi-gration,immunofluorescence for carboxymethyl lysine(CML)clearance and qPCR for sensitive gene mRNA expression.The results showed that the weight-average molecular weight of FMW-SH was 547.7 kDa,the polydispersion coefficient was 17.679,and the cu-mulative transdermal rate within 24 h reached 26.7％,which was significantly better than that of high molecular weight SH and oligo-meric SH.Cytotoxicity tests confirmed the safety of 0.01％～0.5％FMW-SH concentrations.Significant biological activities were ob-served,0.1％～0.5％FMW-SH enhanced typeⅠcollagen synthesis(P<0.05)and keratinocyte migration rate(P<0.05).0.2％FMW-SH reduced CML expression by 52.92％,outperforming aminoguanidine remarkably.0.0005％FMW-SH effectively suppressed IL-4/IL-13induced upregulation of CAII and NELL2 mRNA(P<0.01).FMW-SH can increase the content of typeⅠcollagen,promote ke-ratinocyte migration,eliminate CML and down-regulate the expression of sensitive genes.
Keywords：Full molecular weight sodium hyaluronateTransdermal penetrationType I collagenScratch assayCarboxymethyl lysine
Publication Date：2025-10-30
Online Publishing Date：2026-09-11(First online date of this platform, not the publication date of the document)
Pages：8( 327-334 )