A multiscale blood flow assessment method integrating microscopic dynamic imaging and diffuse correlation spectroscopy
WU Xusheng
WANG Junqi
XU Shuning
SUN Wangwei
GUI Zhiguo
SHANG Yu
Abstract：To enhance the precise application of diffuse correlation spectroscopy(DCS)in medicine,we combined microscopic red blood cell motion imaging with DCS measurement to explore the connection between microscopic red blood cell motion parameters and macroscopic blood flow parameters.Firstly,the sheep blood samples were systematically analyzed through high-speed microscopic ima-ging and DCS.Then,the red blood cell movement parameters were obtained by continuous microscopic imaging and inter-frame corre-lation analysis of images,the mean square displacement(MSD)and diffusion coefficient of red blood cells were determined.Finally,the tissue blood flow index(BFI)was measured by DCS.The results showed that in the experiments of sheep blood samples at different dilution levels,with the increase of the dilution level,the diffusion coefficient of red blood cells in sheep blood increased.At the same time,the BFI measured by DCS showed an upward trend,indicating that the freedom of movement and diffusion coefficient of red blood cells have been significantly enhanced.Among them,at the moderate dilution level,the red blood cell diffusion coefficient was signifi-cantly positively correlated with the BFI measured by DCS(maximum r=0.63,P<0.001).This method can provide an experimental basis for understanding the mechanism of action between microcirculation disorders and ischemic stroke,cardiovascular diseases and the tumor microenvironment.
Keywords：Diffuse correlation spectroscopyRed blood cellsDiffusion coefficientImage frame-to-frame correlationBlood flow index
Publication Date：2025-10-30
Online Publishing Date：2026-09-11(First online date of this platform, not the publication date of the document)
Pages：8( 303-310 )