Research on thrombus behavior in microfluidic chip mimicking microvascular network based on Murray's law
ZHANG Yalei
CHANG Liyun
GENG Jinfa
TAO Ling
LI Weitao
YANG Yamin
Abstract：To study the formation and evolution of microvascular embolisms under different flow conditions and their effects on blood flow distribution,we designed and fabricated a microvascular chip based on Murray's law.Firstly,using microfluidic technology,a dynamic blood flow environment simulating physiological conditions was established.Then laser speckle contrast imaging was em-ployed to obtain real-time blood flow field distributions.Finally,by analyzing the dynamic behavior of thrombus emboli and their im-pact on blood flow distribution,combined with hemodynamic simulation results,the role of hemodynamic factors on embolization was revealed.The results showed that microvascular bifurcation vertices,where blood flow velocity changed and wall shear stress decreased,tended to facilitate thrombus accumulation and growth.When emboli reached a critical size,they might detach and cause mechanical occlusion in downstream branches.Although collateral circulation could maintain the stability of outlet flow velocity in the case of in-complete obstruction,increased flow velocity in collateral vessels could still lead to vascular wall damage and impair local tissue blood exchange efficiency.This research provides a novel technical platform for investigating the mechanisms of microvascular embolism,and holds potential clinical value for the treatment and prevention of microvascular thrombosis.
Keywords：Microvascular chipMurray's lawMicrovascular embolismHemodynamicsLaser speckle contrast imaging
Publication Date：2025-02-28
Online Publishing Date：2026-09-11(First online date of this platform, not the publication date of the document)
Pages：9( 37-45 )