The effect of update frequency on preservation of articular cartilage in vitro
HAN Yunning
QI Jianhong
QU Pengwei
ZHANG Kaihong
BI Yikang
ZHOU Lu
SONG Hongqiang
XIE Di
Abstract：To investigate the effect of the update frequency on the preservation of cartilage in the articular cartilage.Furthermore, to provide an effective experimental basis for the preservation of the osteochondral allograft.60 pieces of cylindrical bone cartilage ob-tained from the lateral loading area of the femoral condyle from the pig knee joint in the aseptic conditions were randomly divided into 3 groups: group A (don't change liquid); Group B (exchange of liquid per 7 days); Group C (exchange of liquid per 2 days).The survival of chondrocytes, histological (Safranine O staining) and biomechanical properties were examined after 28 d preservation.In the 28th d, the survival rate of chondrocytes in each group was decreased ,the survival rate of chondrocytes in group C was better than group A and B (P＜0.05); About Proteoglycan content, group C was higher than group A and group B (P＜0.05),group C was high-er than group A and group B on Young's modulus(P＜0.05).It shows that it is better to replace the preservation solution every two days when use the tissue culture method to preserve articular cartilage.Increase the update frequency of the preservation solution,which can maintain the nutrient content in the preserved solution and provide more adequate nutrition for the osteochondral chondrocytes metabolism.Furthermore, it improves the preservation effect of the osteochondral allografts.
Keywords：Update frequencyTissue cultureTissue bankArticular cartilageOsteochondral allografts
Publication Date：2018-01-01
Online Publishing Date：2026-09-11(First online date of this platform, not the publication date of the document)
Pages：5( 200-204 )