HIV P55-gag Protein Promote Bone Marrow Mesenchymal Stem Cell Senescence
YUAN Yahong
ZHAO Shanshan
WANG Xiaoli
TENG Zhiping
LI Dongsheng
ZENG Yi
Abstract：To explore the influence of HIV P55-gag protein on senescence of bone marrow mesenchymal stem cells, further clarify the mechanism of BMSC osteogenetic differentiation inhibited by HIV P55-gag protein.The BMSC were primary isolated, cultured and purified, then they were divided into two groups: one group was conventionally cultured (BMSCcon) and another was cultured with medium extra added HIV P55-gag protein (BMSCgag).When the BMSCcon and BMSCgag were culture 5, 10, 15 and 20 d, the population doubling level(PDL) were calculated.BMSCcon and BMSCgag cultured 20 d were marked Brdu, then the Brdu incorporation rates were detected with immunofluorescence.The cellular senescence biomarker β-galactosidase of two group cells were detected.The expression intensity of senescence related protein P21 were detected with Western blot.Finally, the two group cells were induced to osteogenesis differentiation.At 15 d and 20 d, the PDL of BMSCgag were significantly lower than that of BMSCcon.The Brdu incorporation rates of BMSCgag were significantly lower than that of BMSCcon, (26±4.1)% and (45±3.7)% respectively.Aging cells of BMSCgag were significantly more than that of BMSCcon,(21±4.7)% and (8±2.1)% respectively.The P21 protein expression intensity of BMSCgag was significantly stronger than that of BMSCcon.Both the Alizarin red and ALP staining showed that osteogenetic differentiation ability of BMSCgag was significantly weaker than that of BMSCcon.The HIV protein P55-gag promotes human bone marrow mesenchymal stem cell senescence and then inhibits osteoblastic differentiation.
Keywords：HIVP55-gag proteinBone marrow mesenchymal stem cellSenescenceOsteogenetic differentiationBone metabolism
Publication Date：2017-01-01
Online Publishing Date：2026-09-11(First online date of this platform, not the publication date of the document)
Pages：6( 52-56,61 )